Cell Line Development Research Associate
Dawar Consulting, Inc.
South San Francisco, CA, USA
6/14/2022
Science
Full Time
Job Description
Job Description
Our client, a world leader in diagnostics and life sciences, is looking for a "Cell Line Development Research Associate” based out of South San Francisco, CA.
Job Duration: Long term Contract (Possibility Of Further Extension)
Pay Rate: $42/hr on W2
Company Benefits: Medical, Dental, Vision, Paid Sick leave, 401K
Cell Line Development Research Associate is involved in the creation of stable cell lines for the recombinant adeno-associated viral vector (rAAV) production processes . He/she executes experiments, produces relevant documentation, and works under supervision.
Responsibilities:
- Culturing mammalian cells for viral production in shake flasks, spinner flasks, spin tubes, deep well and automation platforms.
- Performing experiments in the development, optimization and scale-up of AAV producer cell lines.
- Preparing process development protocols, data analysis summaries, technical reports, and other relevant process documentation.
- Optimization of transfection and transduction protocols for newly engineered cell lines.
- Maintaining an accurate laboratory notebook.
- Ensuring work conforms to applicable quality and safety standards.
Qualifications:
- B.S. with 3-6 years of experience, MSc with 2-5 years of relevant scientific or technical experience in the life sciences industry or equivalent
- Experience in cell culture including materials, instruments and technologies is essential.
- Experience with viral vector production is preferred.
- Hands-on experience in mammalian cell culture production processes is a critical requirement. This includes small scale (shaker flasks, spinner flasks, spin tubes, deep well) and/or bench top bioreactor scale.
- Experience with creation, analysis and characterization of new engineered cell lines and stability studies (sub cloning, clonal expansion, transient transfection and expression screening) is a strong plus.
- Experience in molecular biology techniques (plasmid design, molecular cloning, SDS
- PAGE/western blotting, qPCR, etc) is a strong plus.
- Automation and ELN experience is a strong plus.
- Experience in laboratory automation hardware and software is a plus.
- Due to the inherent nature of cell culture, some weekend work and/or work on holidays may be needed. On occasion, long work hours may be required depending upon the experiment schedule.
- Handling large volumes of cell culture or related materials, such as tens of liters, may be required. Also, several viral materials, including AAV may be involved in the work.
If interested, please send us your updated resume at
hr@dawarconsulting.com/akansha@dawarconsulting.com