Job Description
Job DescriptionQualifications
- PhD in Biochemistry, Biology, Chemistry, Chemical Biology with minimally 2 years of experience in targeted mass spectrometry-based proteomics in industry or startup biotech setting or MS with 5 + years of experience
- Broad experience in targeted MS is required
- Hands on experiences on targeted MS method development and execution, including highly multiplexed and sensitive PRM stable isotope labeled peptide assays using DDA and DIA data
- Hands on experiences in operating, maintaining and troubleshooting nanoLC and Orbitrap MS instruments
- Hands-on experience in sample preparation method development and optimization from various matrices (cells, tissues, plasma, biofluids)
- Demonstrated ability to independently analyze, and interpret targeted proteomics data, and communicate effectively in a fast paced, team-oriented environment
- Experiences in expression proteomics and chemoproteomics are highly desirable
- Demonstrated ability to develop methods and lead mechanism of action mass spectrometry studies
- Develop and optimize mass spectrometry screening assays based on project needs
- Experience with sample preparation from primary cells is a plus
- Motivated problem solver with attention to details, great organization skills and time management, and ability to multitask efficiently to meet drug discovery timelines, team player
Responsibilities
- Develop, optimize, and implement targeted proteomics analysis workflows, including automated enrichment strategies, for precise, accurate, and sensitive protein measurements using stable isotope dilution and label-free mass spectrometry
- Coordinate with project teams to identify targeted proteomics assay requirements, timing, samples and deliverables
- Perform targeted protein MS assays using state-of-the-art LC-MS systems; including nano-LC, Orbitrap MS platforms to determine protein concentrations in cells or tissues
- Prepare samples from a wide variety of biological matrices (cultured cells, tissues, plasma, biofluids) from human and preclinical species using manual and automated bead-based workflows
- Perform data analysis utilizing a range of proteomics software (Skyline, MaxQuant, DIANN, Spectronaut)
- Maintain and qualify HPLC and MS instrument performance
Requirements
- PhD in Biochemistry, Biology, Chemistry, Chemical Biology with minimally 2 years of experience in targeted mass spectrometry-based proteomics in industry or startup biotech setting or MS with 5 + years of experience
- Experiences in expression proteomics and chemoproteomics is a must
Broad experience in targeted MS is requiredPay-$55-65 per hour